Certified Safety Professional Exam 2026 – 400 Free Practice Questions to Pass the Exam

Class 2, Type B3 biosafety cabinets utilize HEPA downflow air, which is crucial for maintaining a sterile environment while working with biohazardous materials. These cabinets are specifically designed with high-efficiency particulate air (HEPA) filters that capture contaminants from the air, ensuring that any airborne particles, including pathogens, are removed before the air is recirculated.

In a Type B3 biosafety cabinet, the air is brought into the cabinet through a HEPA filter, which creates a downward flow of filtered air over the work surface and exhausts to the outside via an additional HEPA filter. This configuration not only protects the operator from potential exposure to harmful agents, but it also prevents the escape of contaminants into the surrounding environment.

The use of downflow air in these cabinets is critical for assuring a high level of safety against pathogens, providing a contained work area where aerosols and particulate matter are effectively controlled and filtered out.